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Grammostola pulchripes
http://atshq.org/boards/viewtopic.php?f=42&t=26316
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Author:  christian elowsky [ Tue Sep 07, 2010 11:18 pm ]
Post subject:  Grammostola pulchripes

Lately many people have shared some wonderful images of tarantulas. Not to be out done, and to keep myself out of trouble, I took an exuvium from a 3" G. pulchripes and took a few pictures.

Everybody loves "eyes":
Image


Here is a little higher magnification. Notice how the eyes rise up out of the surrounding exoskeleton:
Image




Another favored image is that of the "feet" or tarsal pads:
Image


Upon closer inspection, I noticed that not only were the setea numerous,but also covered in fine "hairs", it is no wonder these things are able to walk on water!:
Image




How about a fang and cheliceral teeth?
Image


And finally, this is a shot of the inside surface of one of the leg segments. Notice the pores which would connect to sensory setae? I've seen the membranous filaments before, but can not nail down what they are, possibly the remains from the musculature?
Image


In any case, macrophotography is the way to go, over SEM, unless you want to look at setae and pores... these critters are just too big!

Author:  Mike "troll" Dame [ Wed Sep 08, 2010 12:31 am ]
Post subject:  Re: Grammostola pulchripes

correct the fibers inside are whats left over from musculature

Author:  Anubis [ Wed Sep 08, 2010 2:54 am ]
Post subject:  Re: Grammostola pulchripes

Having access to an SEM is a bit unfair, don't you think? Seriously, though, love the shots, especially the setae.

Author:  christian elowsky [ Wed Sep 08, 2010 4:52 am ]
Post subject:  Re: Grammostola pulchripes

I know it's not quite fair, but hey, when you've got the toys...


It took me roughly an hour to go from exuvium to those few shots. Basically to get any decent resolution, you must coat the samples with various conductive metals. The very bright white ends of the setae is actually electron build up, it's called "charging". To get higher magnifications, I would need to critical point dry the samples with liquid carbon dioxide, and coat for longer. Next time maybe.

Christian

Author:  Anubis [ Wed Sep 08, 2010 5:37 am ]
Post subject:  Re: Grammostola pulchripes

It would definitely be interesting to see them in even higher resolution.

You know what I'd want to see? A Ceratogyrus species' horn in a micrograph. I've always wondered what the structure of that looks like in comparison to the rest of the exoskeleton.

Author:  christian elowsky [ Wed Sep 08, 2010 7:08 am ]
Post subject:  Re: Grammostola pulchripes

Get me an exuvium and I'll do it! As far as I'm concerned, I'll shoot anything I can.

Author:  Ollie [ Wed Sep 08, 2010 6:35 pm ]
Post subject:  Re: Grammostola pulchripes

Interesting shots Christian but only monochrome? :razz:

Seriously though maybe when I get the photo galleries caught up we can have a section for close up macro shots (like Anubis's claw shots) and SEM's etc.

Author:  christian elowsky [ Wed Sep 08, 2010 6:49 pm ]
Post subject:  Re: Grammostola pulchripes

As I said, I have access to the tools and to sputter coat and shoot eye turrets is easy as pie. I am working up another leg for better setae images.

As for color, all colorized SEM's are done in two ways, post acquisition psuedocoloration, or there are systems which psuedocolor while the SEM is acquring the image. The latter is quite expensive, but they are impressive.

I might try CS3 to give it a go...

Christian

Author:  christian elowsky [ Wed Sep 08, 2010 7:00 pm ]
Post subject:  Re: Grammostola pulchripes

ImageJ, loaded LUT (look up table), edited the brightness and contrast.


Image

Author:  zonbonzovi [ Thu Sep 09, 2010 2:04 am ]
Post subject:  Re: Grammostola pulchripes

Ollie wrote:
Interesting shots Christian but only monochrome? :razz:

Seriously though maybe when I get the photo galleries caught up we can have a section for close up macro shots (like Anubis's claw shots) and SEM's etc.


Man, I love SEM photos. Very cool, Christian! It would be great to see Ollie's suggestion used in conjunction with hard to ID genera.

Author:  lilywhirl [ Tue Aug 16, 2011 8:43 pm ]
Post subject:  Re: Grammostola pulchripes

Really cool pictures!!!

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